ADVANCEMENTS IN CRISPR-CAS9 FOR GENETIC ENGINEERING

Authors

  • Hamroyeva Gulshanda Azimovna Polytechnical school No. 1 of Gijduvan district, Bukhara, Uzbekistan

Keywords:

CRISPR-Cas9, genome editing, genetic engineering, gene therapy, agricultural biotechnology, base editing, prime editing, off-target effects, bioethics, synthetic biology

Abstract

This article reviews the transformative impact of CRISPR-Cas9 on genetic engineering, detailing its mechanisms, applications in biomedicine and agriculture, challenges like off-target effects, and future directions. It provides a comprehensive overview for researchers and students interested in genome editing.

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References

Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., ... Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157.

Barrangou, R., & Horvath, P. (2017). CRISPR: New horizons in phage resistance and strain improvement. Annual Review of Food Science and Technology, 8, 363–384.

Cyranoski, D. (2020). CRISPR gene-editing tested in a person for the first time. Nature, 587(7834), 346–347.

Doudna, J. A., & Charpentier, E. (2014). The new frontier of genome engineering with CRISPR-Cas9. Science, 346(6213), 1258096.

Hsu, P. D., Lander, E. S., & Zhang, F. (2014). Development and applications of CRISPR-Cas9 for genome engineering. Cell, 157(6), 1262–1278.

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012). A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity. Science, 337(6096), 816–821.

Wang, H., La Russa, M., & Qi, L. S. (2016). CRISPR/Cas9 in genome editing and beyond. Annual Review of Biochemistry, 85, 227–264.

Zhu, H., Li, C., & Gao, C. (2020). Applications of CRISPR-Cas in agriculture and plant biotechnology. Nature Reviews Molecular Cell Biology, 21(11), 661–677.

Qudratova, G. M. (2025). MINTAQAVIY ILMIY-TADQIQOT INSTITUTLARI: RIVOJLANISH YO'NALISHLARI VA TAQQOSLAMA TAHLIL.IZLANUVCHI, 1(6), 6-12.

Qudratova, G. (2025). EFFECTIVE USE OF ECONOMETRIC RESEARCH-AS A FACTOR OF ECONOMIC DEVELOPMENT.Journal of Applied Science and Social Science, 1(3), 218-220.

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Published

2025-05-26

How to Cite

Hamroyeva Gulshanda Azimovna. (2025). ADVANCEMENTS IN CRISPR-CAS9 FOR GENETIC ENGINEERING. Journal of Applied Science and Social Science, 15(05), 284–286. Retrieved from https://www.internationaljournal.co.in/index.php/jasass/article/view/1098

Issue

Vol. 15 No. 05 (2025): Journal of Applied Science and Social Science

Section

Articles

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